|
KMID : 0368419820250040161
|
|
Journal of Plant Biology
1982 Volume.25 No. 4 p.161 ~ p.168
|
|
|
Hormonal Effect and Cytokinin Autonomy in Callus Culture of Phaseolus vulgaris L.
|
|
Kim, Sang Gu/金相九
Song, Ju Ho/Lee, Kwang Woong/宋周鎬/李光雄
|
|
|
Abstract
|
|
|
|
The activities of auxins and cytokinins have been examined in the growth of callus tissue derived from Phaseolus vulgaris L. cv. Damyang. The synthetic auxin, picloram was the most effective in promoting callus growth and the range of effective concentrations (0.1 μM to 32 μM) was broad. 2,4-D also enhanced callus growth at the optimal concentration of 3.2 μM. NAA promoted callus growth at relatively higher concentrations than other auxins tested. IAA was less effective in supporting callus growth. Cytokinin bearing saturated side chain (N^6-isopentyladenine) was approximately 30 times more active than the corresponding unsaturated compound, N^6-(Δ^2-isopentenyl) adenine. The abilities of cytokinin-autonomous growth were also examined. Callus tissues previously grown on concentrations lower and/or higher than optimal concentrations of cytokinins were better habituated in the subsequent passage. It was suggested that the development of cytokinin autonomy may be related to dosage-concentrations of cytokinin in the previous passage.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|